Composite

Part:BBa_K912020:Design

Designed by: Grant Nicholas   Group: iGEM12_Northwestern   (2012-10-01)


BBa_J23111(newstrong) promoter/RBS+ Aspergillus Niger Phytase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1410
    Illegal BamHI site found at 590
    Illegal XhoI site found at 113
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

None besides primer design for pcring out the aspergillus niger phytase from the genome


Source

Constitutive promoter: registry Aspergillus Niger Phytase: genomic sequence

References